Identification and characterization of a novel bacteriocin from microbial genome database

Identification and characterization of a novel bacteriocin from microbial genome database

dc.contributor.guide	Sareen, Dipiti
dc.coverage.spatial	Biochemistry
dc.creator.researcher	Mangal Singh
dc.date.accessioned	2023-08-30T05:43:35Z
dc.date.available	2023-08-30T05:43:35Z
dc.date.awarded	2018
dc.date.completed	2017
dc.date.registered	2012
dc.description.abstract	A class of bacteriocins known as lantibiotics, are small ribosomally synthesized and post translationally modified peptides (RiPPs) containing lanthionine rings. In this study, 24 novel putative lantibiotic biosynthetic gene clusters (BGCs) were identified from NCBI genome database. BGC of Streptomyces filamentosus NRRL 11379, encoding Roseocin was characterized by heterologous coexpressíon of the minimally required genes in E. coli. The lanthipeptides were purified to homogeneity, and characterized by mass spectrometry. Antimicrobial activity analysis of activated Roseocin established it to be a novel two component lantibiotic. newline
dc.description.note	Bibliography 151-164p.
dc.format.accompanyingmaterial	CD
dc.format.dimensions	-
dc.format.extent	164p.
dc.identifier.uri	http://hdl.handle.net/10603/509864
dc.language	English
dc.publisher.institution	Department of Biochemistry
dc.publisher.place	Chandigarh
dc.publisher.university	Panjab University
dc.relation	-
dc.rights	university
dc.source.university	University
dc.subject.keyword	Bacteriocin
dc.subject.keyword	Genome database
dc.subject.keyword	Lantibioticy
dc.subject.keyword	Microbial
dc.subject.keyword	Roseocin
dc.title	Identification and characterization of a novel bacteriocin from microbial genome database
dc.title.alternative
dc.type.degree	Ph.D.