Pharmacological Investigation of Oryza sativa Antioxidants in Neurodegeneration

Abstract

i newlineAbstract newlineOryza sativa serves as a staple food worldwide and it is enriched with essential phytophenolic newlinechemical constituents which possess plenty of disease modulating features. and#947;-Oryzanol (OZ) and newlineEllagic acid (EA) are chemically phenolic phytoconstituents having prominent anti-oxidative newlineeffects and have potentials to modulate molecular biochemistry in beneficial way. Hence, current newlineinvestigation was aimed to evaluate neuroprotective and cognitive enhancement effects of Oryza newlinesativa phytophenolic constituents in STZ induced SAD/ LOAD and Aand#946; (1-42) induced EAOD in newlineanimal models. Both these animal models mimic the pathological relevance of neurodegenerative newlineailments as in humans. In silico experiments suggested target specificity of FA, OZ and EA for newlineAchE and Aand#946; (1-42). Our findings suggested the safety and cytoprotective profile of Oryza sativa newlinephytoconstituents in presence of neurotoxins in the SH-SY5Y human neuroblastoma cell line. newlineFurthermore, antioxidative effect by DPPH assay, anti-AchE effect against AchE enzyme from newlineElectrophorus electricus and Aand#946; (1-42) antiaggregatory effects fulfilled prime requirements of newlineOryza sativa phytochemicals to get explored further for neurodegenerative ailments. The in vivo newlineexperiments suggested therapeutic effects of FA, OZ and EA in STZ induced LOAD and Aand#946; (1- newline42) induced EOAD with reference to their biochemical as well as behavioural modulatory features. newline30 days treatment of OZ (100 mg/kg, p.o.) and EA (50 mg/kg, p.o.) in STZ (3 mg/kg i.c.v.) induced newlineSAD showed correction of biochemical abnormalities by improvement in oxidative stress profile, newlineretardation of pro-inflammatory markers i.e. GFAP and CRP, AchE level, and Aand#946; plaque level. newlineTreatment of EA i.e. EA70 and EA140 (single dose of 70 and#956;M and140 and#956;M respectively, i.c.v.) in newlineAand#946; (1-42) (10and#956;L, 1and#956;g/and#956;L, i.c.v.) treated animals signified Aand#946; (1-42) anti-aggregatory effect, Ca+ newline+/Calpain/GSK-3and#946;/CDK5 modulatory, anti-tauopathic effects. Furthermore the study signified newlineanti-inflammatory effects through prevention of astroglial ac