Evaluation of the prevalence of gingipain and fimbriae genotypes of porphyromonas gingivalis in patients with chronic periodontitis and healthy individuals

Abstract

Periodontal disease is a multifaceted, polymicrobial infectious disease newlinecharacterized by gingival tissue inflammation and periodontal ligament and alveolar bone newlinedamage, leading to tooth loss. Porphyromonas gingivalis (P. gingivalis) is considered to newlineplay a major role in periodontal disease pathogenesis and is recognized as a keystone newlinepathogen. The organism P. gingivalis possesses diverse virulence factors among which newlinefimbriae and cysteine proteases (gingipains) are most extensively studied for their role in newlineproviding pathogenic ability to P. gingivalis. The fimbriae play an imperative role in newlinebacterial colonization, adhesion and invasion whereas gingipains are capable of cleaving newlineand degrading host proteins, disrupt complement system and inhibit iron transport. newlineFimbriae are encoded by fimA gene and are classified into six genotypes; type I, Ib, II, III, newlineIV and V. Gingipains are categorized as: Arginine-dependent gingpian R (encoded by newlinergp) and Lysine-dependent gingipain K (encoded by kgp). Three types of rgpA gingipain newline(type A, B, and C) and two types of kgp gingipain (kgp-I, kgp-II) have been identified newlinepreviously. The previous findings imply that there is variable occurrence of these newlinegenotypes across all geographic locations providing differential level of severity in health newlineand disease. We aimed to investigate the occurrence of gingipain and fimbriae genotypes newlineof P. gingivalis and their association with clinical characteristics in patients with chronic newlineperiodontitis (CP) and healthy individuals (H). We also want to investigate the newlinerelationship between the occurrence of gingipain and fimbriae genotypes with quantity of newlineP. gingivalis in subgingival plaque samples of these subjects. newline newline