Investigating the unique features of self assembled hen egg white lysozyme nano aggregates using biophysical approaches

Abstract

We report a novel strategy for synthesis of Hen egg white Lysozyme nanoparticles by alkaline pH induced aggregation at room temperature 298 K Aggregation at alkaline pH is characterized by an absence of lag phase or nucleation phase Thus aggregation proceeds without any requirement of critical concentration of the nucleus These nanostructures offer the advantage of synthesis in a size controlled manner Intermolecular association by disulfide linkage s s renders the protein conformation