Association of IL 17A Gene Polymorphism and Its Influence on Serum IL 17A and IL 6 Levels in Diabetes with Chronic Periodontitis Patients

Abstract

Abstract: newlineIntroduction: newlineThe periodontitis is an inflammatory disease resulting in loss of soft and hard newlinetissues around the tooth.The role of genetic factors in the immune response is newlineimportant for maintenance and progression of diseases.The IL-17 is a group newlineof cytokines which comprises six different molecules (IL-17A, B, C, D, E and F). newlineIn this regard, IL-17A and F are the most commonly understood cytokine which newlineplays an important role in inflammatory diseases and periodontal inflammation. newlineIL-17A polymorphism was associated with chronic periodontitis (CP) and newlineaggressive periodontitis and also frequently associated with in type 1and 2 newlinediabetes mellitus patients and related with metabolic status of diabetes. The newlinepresent study was designed to evaluate whether Interleukin-17A gene newlinepolymorphism has any influence on development of periodontitis in type 1and2 newlinediabetes patients. newlineMaterials and Methods: newlineThis study was done on 359 subjects, and consists of 6 groups and these are newlinegroup 1 which included of 28 patients from type I diabetes with periodontitis. newlineThe group-2 was patients from type 1 diabetes and without chronic newlineperiodontitis, there were 71 type 2 diabetic patients with chronic periodontitis newlinein group 3 while 76 patients were included in group 4 and they had only type 2 newlinediabetes. In the same way, there were total of 78 non diabetic patients in the newlinegroup-5 with only chronic periodontitis. The last group 6 consists of newlinenondiabetic patients and the sample size for this group was 78 patients and newlineno one has periodontitis. Approximately 5 ml of blood was collected under newlinestrict aseptic conditions from the anticubital fossa of each patient. IL-17A newlinepolymorphism was identified by using PCR RFLP (Restriction Fragment newlineLength Polymorphism). Serum IL-17A and IL-6 were analysed by using Enzyme newlineLinked Immunosorbent Assay (ELISA) by sandwich Elisa kit principle. newlineResults: newlineGenotype frequency of all the six groups showed that there is no significant newlinedifference in the genotype frequency with any group in comparison to control