Synthesis and in vitro characterization of cyanoacrylate

Abstract

AIM: newlineTo evaluate the effectiveness of antimicrobial newlineincorporated cyanoacrylate and its comparison to the newlinecommercially available cyanoacrylate. newlineMATERIALS AND METHODS: newline Present study was done in Green lab and White lab newlineat Saveetha Dental College, cyanoacrylate was prepared newlinefollowing all steps in the lab and antimicrobial newlineincorporation was successfully carried out. newlinePreparation of Povidone Iodine newline In 5 ml of dcm and 5 ml of ethanol 0.02gm of newlinePovidone iodine was mixed using vortex. newlinea. Preparation of chlorhexidine newlineIn 5 ml of dcm and 5 ml of ethanol 0.02gm of newlinechlorhexidine was mixed using vortex. newlineb. Preparation of combination newline4 ml of prepared chlorhexidine solution was mixed newlinewith 1 ml of povidone iodine solution to create a 4:1 ratio newlineof the antibiotic. newlineIncorporation of antimicrobial in prepared cyanoacrylate newlinePrepared cyanoacrylate was mixed in 100and#11417;l of DCM and newline50and#11417;l of antibiotic. newlineSAMPLE PREPARATION AND GROUPS newlineThere are totally 6 groups in the study newlineGroup 1: Cyanoacrylate + CHX newlineGroup 2: Cyanoacrylate +PI newlineGroup 3: Cyanoacrylate +CHX+PI newlineGroup 4: Cyanoacrylate only newlineGroup 5: Lab Made Cyanoacrylate newline newline4 TEST WERE DONE USING THESE SAMPLES newline Antimicrobial Testing newline FTIR newline Tensile Strength Testing newline Cell Culture for Cytotoxicity newlineRESULTS newline FTIR testing showed successful antimicrobial newlineincorporation in cyanoacrylate. There is a significant newlinedifference between the control group and the other newlineantimicrobial group (plt0.05). Although the tensile newlinestrength was more for the control group compared to the newlineother groups (plt0.05), it was not significant for group 4 newlineand group 2 (pgt0.05). For cytotoxicity testing using MTTA newlineassay when the control group was compared with the newlineother groups then the results were statistically not newlinesignificant (pgt0.05). newlineThis study concludes that cyanoacrylates as a pure newlinemolecule shows very less antimicrobial activity which is newlineenhanced on addition of antimicrobials, but tensile newlinestrength reduces as the molecule becomes diluted