Phytochemical Screening Antimicrobial and Anti Acne Potential of Tinospora Crispa

Abstract

ABSTRACT: newlineThe plant screening for phytochemical constituents seems to have the potential to act as a source of useful drugs and cures many infections as a result of the presence of various bioactive compounds that evident to have enormous activity against array human pathogens. The objective of the study was to undertake a comparative qualitative phytochemical analysis of the different parts of Tinospora crispa (T. crispa), a traditional herb used against several diseases. The different parts of T. crispa were extracted using maceration method. The results of phytochemical screening indicated that T. crispa contains alkaloids, flavonoids, tannins, saponins, steroids and terpenoids. The TLC profiles of samples, depicted through the Rf values of resolved compound bands and the solvent system selected for the best results of TLC was Toluene: Ethyl acetate: Formic acid (7:5:1) and (5:4:1) for gallic acid and quercetin respectively. Total phenol, flavonoids and alkaloid content was estimated following standard procedures already reported. Antioxidant activity was assessed by using 2, 2- diphenyl-1-picryl-hydrazyl (DPPH) assay using ascorbic acid as standard antioxidant and antimicrobial activity were calculated by the disc diffusion method. The total phenolic content in leaves and flower extract was found to be 3.600 and 2.044 in mg/g equivalent to gallic acid. The total flavonoid content in leaves and flower extract was found to be 2.491 and 1.258 in mg/g equivalent to quercitin. The total alkaloid content in leaves and flower extract was found to be 3.228 and 0.847 in mg/g equivalent to atropine. IC50 for standard ascorbic acid was found to be 17.68and#956;g/ml and for hydroalcoholic extract of leaves and flower was found to be 57.17 and#956;g/ml and 94.13 and#956;g/ml respectively. Antimicrobial activity results suggest that Tinospora crispa is a potential source of broad-spectrum antimicrobial agents. The acne like inflammatory activity was carried out by measuring the ear thickness. Flower ethanolic extract of Tinospora crispa showe