Essential oil bioactives terpinen 4 OL and and#945;terpineol exploration of a new horizon in combating Pseudomonas Aeruginosa induced keratitis

Abstract

The present study was aimed to explore the antivirulence and antibiofilm effects of essential oil components, Terpinen-4-ol and and#945;Terpineol against Pseudomonas aeruginosa and to further investigate the therapeutic efficacy of selected component in treating Pseudomonas aeruginosa induced keratitis in murine model. Terpinen-4-ol and and#945;-Terpineol suppressed virulence phenotypes and biofilm formation successfully. The in vitro results were confirmed by molecular docking analysis and gene expression profiles. Further, it was observed that nanoencapsulation of selected and#945;-Terpineol offered enhanced activity over free form in reducing virulence traits of Pseudomonas aeruginosa. Apparently, nanoencapsulated and#945;Terpineol (and#945;T-NLCs) may serve as promising antivirulence agent against Pseudomonas aeruginosa induced keratitis. The present study thus opens up a vast area for using and#945;-Terpineol as potent antivirulence drug candidate to treat Pseudomonas aeruginosa associated infections. newline