A study of genetic predisposition and skin microbiome in patients with Psoriasis attending a tertiary care hospital

Abstract

Psoriasis is a non-contagious inflammatory skin disorder characterized by sharply marginated raised scaly, red indurated erythematous plaques. Based on clinical appearance, disease has been classified into Plaque psoriasis, Guttate psoriasis, Generalized pustular psoriasis, Erythrodermic psoriasis and Psoriatic Arthritis. Individuals with HLA class I and II genes have been reported to have higher risk of getting psoriasis. newlineAim: newlineTo study the genetic predisposition and skin microbiome in patients with psoriasis attending a tertiary care hospital. newlineMaterials and methods: newlineA case control study was carried out over a period of 3 years (from August 2015 to July 2018) at Chettinad Hospital and Research Institute. A total of 228 Psoriasis cases and 242 healthy controls were enrolled in the study. The frequency and association of HLA alleles (Cw*06:02, DRB1*07:01, DRB1*08:02, DQB1*03:03) in psoriasis patients and healthy controls was studied by Multiplex PCR with Sequence Specific Primers. newlineResults: newlineThe allele frequencies of HLA Cw*06:02, DRB1*07:01, DQB1*03:03 alleles in the psoriasis patients were significantly higher than those in the healthy control group; there was a significant correlation between them. The frequency of HLA Cw*06:02, DRB1*07:01, DQB1*03:03 alleles were significantly associated with type I psoriasis. The frequency of HLA DQB1*03:03 allele (P= 0.004) showed statistically significant association in psoriasis patients with family history of psoriasis. newlineConclusion: newlineWe found that HLA Cw*06:02, DRB1*07:01, DQB1*03:03 were positively associated with psoriasis where the frequency of these alleles were higher in females. newline newline