PREPARATION AND PHARMACOLOGICAL EVALUATION OF POLYHERBAL FORMULATION FOR THE MANAGEMENT OF ALOPECIA AND INVESTIGATION OF CHEMICAL CONSTITUENTS RESPONSIBLE FOR SUCH INDICATION

Abstract

ABSTRACT newlineA wide effect (or side-effect) of chemotherapy or radiation therapy in cancer patient is the attest of growth of dividing cancer cells, however the non-discriminatory effect of the therapy on diving cells especially in gastrointestinal tract, bone marrow and hair follicle. Our aim is to investigate the protective effect of isoflavonoids, especially chemotherapy, radiation and stress Induced alopecia. Isoflavonoids increasing the release of calcitonin gene related peptide (CGRP) and insulin growth factor (IGF-I) level and these might be increase the possibilities that promote hair follicle growth in radiation hair loss. IGF-I and CGRP release, is increased by soya isoflavone which show vasodilatation effect in hair follicle. Flavonoids (procynidine) and isoflavonoids (Genistein and daidzein) reduce dihydroxytestosterone(DHT) level in serum which is responsible for alopecia. The present study aimed at hair growth activity of Pueraria tuberosa, Cratageus oxycantha, Vigna radiata and Momordica charantia by using cyclophosphamide, Radiation and stress induced alopecia and modulating the mechanisms responsible for this condition. The hypothesis was undertaken to verify this by exposing C57BL/6 mice to alopecia. To evaluate the effect of hair loss on number of hair follicle, hair follicle number and hair density in mice was measured with biochemical markers like Lymphocyte cell, Alkaline phosphate, Alkaline transferase and cholesterol. The result of our analysis suggest that polyherbal formulation followed by Cratageus oxycantha extract and Pueraria tuberosa extract shows best result with high hair density and hair follicle number as well as low lymphocyte count. Result supported that significant change of biochemical markers i.e. alakaline phosphate, cholesterol and alanine transferase in peripheral blood. The anti-alopecic activity was further supported by histopathological studies of hair follicle which show significant result of hair follicle as compared to radiation treated hair follicle group. Isoflavonoi