Development And Evaluation of Lactoferrin Modified Nano emulsion Of Posaconazole for Ocular Delivery

Abstract

The eye is specialized part of body comprised of numerous physiological barriers that limit the drug absorption at action site. Among all fungal eye infection considered serious. So the aim of study was to develop lactoferrin modified nanoemulsion of posaconazole for ocular delivery. Based on solubility enhancement techniques nanoemulsion was selected on the basis of its smaller size, non-toxic, ease of applicability ,also improve better uptake of oil soluble component. Lactoferrin is a belonging to transferrin family of proteins and reported for dry eye syndrome. This protein molecule even a low concentration increases cellular uptake with all desirable quality control attributes. In nanoemulsion formulation, based on solubility study Isopropyl myristate (Oil), Tween 40 (surfactant), PEG 200 (Co-surfactant) were selected. Maximum emulsification region was selected by plotting pseudo ternary diagram with 2:1 ratio of surfactant and cosurfactant. Optimization of formulation was done by using central composite design. Lactoferrin loaded nanoemulsion prepared by loading of aqueous solution of lactoferrin to optimized nanoemulsion. 0.25% concentration of lactoferrin meet all quality attributes like targeted particle size, in-vitro drug release, polydispersity index, viscosity etc. The surface morphology and particle size was confirmed by transmission electron microscopy analysis. Based on minimum inhibitory concentration study, lactoferrin loaded drug showed higher zone of inhibition as compared to marketed and conventional prepared nanoemulsion. Hen s egg test chorioallantoic membrane study and osmolarity value of lactoferrin loaded nanoemulsion indicated safe and no any signs of irritation. No any interaction by drug and excipient was confirmed by high performance liquid chromatography. Non-fickian drug release by goodness % fit to kosmeyer peppas model was followed. Cytotoxicity study by neutral red uptake assay and cellular uptake study demonstrated low IC50 value and higher cellular uptake as compared Noxafil