Bioprospecting Potential Biomolecules against Microbial infections

Abstract

The present research reveals about the antimicrobial potential of a bacteriocin from an newlineethnomedicinal plant originated strain of Bacillus subtilis (MK733983). Some ethnomedicinal newlineplants were screened for microbes that can produce anti-mycobacterial bacteriocin and have newlinesuccessfully isolated such a microbe. Based on16S rRNA sequencing, the isolated microbe newlineshowed a 100% similarity index with Bacillus subtilis and it was assigned as MK733983. The newlineoptimization of the microbe enhanced the bacteriocin activity by 33% and showed many stable newlinecharacteristics. The bacteriocin was purified using RP-HPLC and elution with 65% acetonitrile newlineshowed highest antimicrobial activity with Mycobacterium smegmatis. Its specific activity and newlinepurification fold increased by 70.5% and 44%, respectively, compared to crude. Its molecular newline newlineweight was estimated to be 3.4KDa by LC-ESI-MS/MS analysis. The bacteriocin is non- newlinehemolytic and exhibited a broad inhibition spectrum with standard strains of Staphylococcus newline newlineaureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli and newlineChromobacterium violaceum. Scanning Electron Microscopy showed cell annihilation with newlinepores in cell membranes of S. aureus and P. aeruginosa treated with the bacteriocin, newlineimplicating bactericidal mode of action. Antibiofilm analysis with 0.5-3x minimal bacteriocin newlineconcentrations with prioritized standard strains has shown significant biofilm inhibition and newlineeradication. Additionally, the antibiofilm efficacy was confirmed with the scanning electron newlinemicroscope observations. It exhibited remarkable synergistic potential (SP) with 9 front line newlineantibiotics. MTT assay on 3T3 cell lines of bacteriocin revealed that it is non-cytotoxic and newlinewas reconfirmed by trypan-blue staining. Many deleterious infections are caused by biofilm newlineformers instigating antimicrobial resistance and there is an escalating demand. Bacteriocins are newlinepromising antibiotic alternatives and the findings of the present study are competent to be newlineconsidered.