Role of Sex Determining RegionYin EpithelialMesenchymal Transitionin Cervical Cancer

Role of Sex Determining RegionYin EpithelialMesenchymal Transitionin Cervical Cancer

dc.contributor.guide	Pawan K. Jewaria
dc.coverage.spatial
dc.creator.researcher	Rukhsar Fatima
dc.date.accessioned	2019-09-17T05:53:54Z
dc.date.available	2019-09-17T05:53:54Z
dc.date.awarded	2017
dc.date.completed
dc.date.registered
dc.description.abstract	Background: Breastcancerisoneoftheleadingcauseofcancer-relateddeathsinwomenworldwideandincreasing newlinerapidlyindevelopingcountries.Inthepresentstudy,weinvestigatedthepotentialroleandassociationofHSP70-2 newlinewithbreastcancer. newlineMethods: HSP70-2expressionwasexaminedin154tumorand 103adjacentnon-canceroustissue(ANCT) newlinespecimens andbreastcancercelllines(MCF7,BT-474,SK-BR-3 andMDA-MB-231)byRT-PCR,quantitative-PCR, newlineimmunohistochemistry,Westernblotting,flowcytometryandindirectimmunofluorescence.Plasmiddriven newlineshort hairpinRNAapproachwasemployedtovalidatetherole ofHSP70-2incellularproliferation,senescence, newlinemigration,invasionandtumorgrowth.Further,westudiedtheeffectofHSP70-2proteinablationonsignaling newlinecascadesinvolvedinapoptosis,cellcycleandEpithelial-Mesenchymal-Transitionbothincultureaswellasin-vivo newlinehumanbreastxenograftmousemodel. newlineResults: HSP70-2expressionwasdetectedinmajorityofbreastcancerpatients(83%)irrespective ofvarioushistotypes, newlinestagesandgrades.HSP70-2expressionwasalsoobservedinallbreastcancercells(BT-474,MCF7,MDA-MB-231and newlineSK-BR-3)usedinthisstudy.DepletionofHSP70-2inMDA-MB-231andMCF7cellsresultedinasignificantreductionin newlinecellular growth,motility,onsetofapoptosis,senescence, cellcyclearrestaswellasreduction oftumorgrowthinthe newlinexenograft model.Atmolecularlevel, down-regulation ofHSP70-2resultedin reducedexpressionofcyclins,cyclin newlinedependentkinases,anti-apoptoticmoleculesandmesenchymalmarkersandenhancedexpressionofCDKinhibitors, newlinecaspases,pro-apoptoticmoleculesandepithelialmarkers. newlineConclusions: HSP70-2isoverexpressedinbreastcancerpatients and wasinvolvedinmalignantpropertiesofbreast newlinecancer. ThissuggestsHSP70-2maybepotentialcandidatemolecule fordevelopmentofbetterbreastcancertreatment
dc.description.note	HSP70-2,Breastcancer,Genesilencing,Apoptosis,Tumorgrowth
dc.format.accompanyingmaterial	DVD
dc.format.dimensions
dc.format.extent
dc.identifier.uri	http://hdl.handle.net/10603/257686
dc.language	English
dc.publisher.institution	Department of Life Sciences
dc.publisher.place	Jaipur
dc.publisher.university	Jaipur National University
dc.relation
dc.rights	university
dc.source.university	University
dc.title	Role of Sex Determining RegionYin EpithelialMesenchymal Transitionin Cervical Cancer
dc.title.alternative
dc.type.degree	Ph.D.