Optimization of high cell density cultivation for the production of recombinant filarial protein wbsxp1 and diagnostic application in human lymphatic filariasis

Abstract

Human Lymphatic filariasis is a major debilitating disease affecting newline120 million over 80 countries This is a mosquitoborne parasitic disease newlinecaused by three species of tissue dwelling filaroid nematodes Wuchereria newlinebancrofti is responsible for 90 of the cases and is found throughout the newlinetropics and in some subtropical areas worldwide newlineAs part of the WHO sponsored Filarial Genome Project many novel newlinegenes of Wbancrofti and Bmalayi important for their diagnostic newlineprophylactic chemotherapeutic potential and other genes involved in immune newlineevasion and parasite survival mechanisms have been identified from various newlinestages of the parasite life cycle Wuchereria bancrofti protein WbSXP1 was newlineidentified and established as a potential candidate for the diagnosis of newlinelymphatic filariasis newline newline