Interaction of Bovine Serum Albumin with Nitrogen based Organic Compounds Study of Spectroscopic and Molecular Docking

Abstract

newline The most prevalent proteins in the blood circulation system are serum albumins. They newlinehave significantly contributed to the spread of numerous medications to numerous newlinedifferent disease areas. The main ingredient in blood plasma, serum albumin, is in newlinecharge of transporting both exogenous and endogenous chemicals to their intended newlinesites. The binding affinity between drugs/ligands and serum albumin plays a newlinesignificant role in designing novel drugs/ligands and altering the doses of pre-existing newlinedrugs/ligands to achieve the desired therapeutic efficacy. The interaction between newlinebovine serum albumin (BSA) and nitrogen based ligands play important role in newlinebiological, pharmaceutical and environmental applications. The nitrogen based newlineligands were designed, synthesized and characterized using various spectroscopic newlinetechniques such as FTIR, MS, 1H-NMR and 13C-NMR. The nature and binding newlineinteractions of the ligand and BSA have been explored using UV-Vis absorption newlinespectroscopy, fluorescence spectroscopy, and molecular docking analysis. Resistance newlineto antibiotics/ antibacterials/ antifungals in pathogenic microbes has been developing newlineover the past few decades and has recently become a commonplace public-health newlineperil. Thus, alternative nontoxic potent antibiotic agents are covetly needed to control newlineantibiotic-resistant outbreaks. Therefore, anti-cancer, anti-microbial application has newlinebeen explored using cancer cell and infected pathogens. Finally, designed ligands newlinewere employed to detect trace amount of water in various organic solvents. newlineKeywords: Nitrogen based ligands; Bovine Serum Albumin; UV-Visible; newlineFluorescence; Fluorescence Anisotropic; Fluorescence life-time; Anti-cancer; Anti- newlinemicrobial; Fluorescence chemosensor; Molecular Docking