Isolation and Characterization of Stromal Vascular Fraction from Adipose derived stem cells and their potential application in treating chronic ulcers

Abstract

Chronic ulcers affects quality of life and results in economic and social burdens. Local newlinetransplantation of human adipose-derived stem cells (ADSCs) is considered an effective newlinetreatment. However, ADSC suspension alone is vulnerable to the immune system and newlineresults in a shortened cell survival. There is increasing evidence of a synergistic effect of newlineplatelet-rich plasma (PRP) combined with ASCs on wound healing, and the combination newlineof ADSC s with hydrogels on accelerating wound healing. This thesis investigated the newlineeffectiveness, of wound healing following local application of a) PRP combined with newlineADSCs in a rabbit ear pinna model, and b) Local application of a alginate hydrogel newlineembedded with AD-MSC s . Standard of care was local application of povidine iodine newlinesolution as the control intervention. Wound healing by wound contraction, epithelialisation newlinewere investigated. Chronic ulcer healing was promoted by the synergistic effects of PRP newlinecombined with ADSCs and the Alginate Hydrogel embedded AD-MSCs in a bandage newlineform. The combination was more effective than the standard of care. The data provides a newlinefoundation for the clinical administration of ADSCs combined with PRP and alginate newlinehydrogels in chronic ulcer healing and skin regeneration. newlineKey Words: Adipose derived stem cells (ADSCs), Platelet Rich Plasma (PRP), Chronic newlinewounds, Alginate Hydrogel newline