A COMPARATIVE COMPREHENSIVE TRANSCRIPTOME SEQUENCE ANALYSIS FOR VARIOUS STRESS RESPONSIVE FACTORS IN LEGUMES

Abstract

Legume is one of the worldand#712;s most important crop, it is consumed worldwide and newlineplay a significant role in the economy of producing countries. Legume crop productivity is newlineseverely affected due to various stress factors both biotic and abiotic (e.g. drought, salinity, cold, newlineethylene temperature, kinase cascade) these stress factors both biotic and abiotic significantly newlineimpact the production of crop in response to these, plants activates a number of defense newlinemechanism that function to increase tolerance in adverse conditions. Therefore tracking the newlinecandidate genes responsible for stress tolerance through sequence similarity and functional newlinestudies is becoming increasing important for breeding and will act as useful resource for newlinecomparative genomics and can be further used as molecular markers or for genetic newlinetransformation to develop desired. Thus, research initiatives have been launch to produce newlinegenomic and transcriptomic data about legumes species (Cajanus cajan, Cicer arietinum, Pisum newlinesativum and Lens culinaris). newlineResults: Assembling the ESTs of Cajanus cajan, Cicer arietinum, Pisum sativum, Lens culinaris newlineand by applying various bioinformatics tools, the detection of GC content through GC profile, newlineprediction of ka/ks values using MEGA 5.0, identified legume genes under purifying and newlinepositive selection and phylogenetic relationship among various stress factors(biotic and abiotic) newlinein four legume species. On these result we emphasize gene annotations and discuss various stress newlinefactors based on the categories defined.. The availability of plant genome data has stimulated us newlineto select two candidate gene for In silico homology modelling of plant proteins which are newlinestructurally unknown. In this study physico-chemical and functional characterization of GAPDH newlineand 14-3-3 protein in Cajanus cajan, Cicer arietinum, Pisum sativum and Lens culinaris were newlinedetermined.