Bioactivity guided isolation characterization and estimation of phytoconstituents from leaves and roots of launaea pinnatifida cass a species of the controversial drug gojihva

Abstract

Background: Loss of expertise in Sanskrit language and medicinal plant identification as per Ayurvedic texts has resulted in the creation of Controversial Drugs, i.e., plants with different botanical identity being used by the same name. Launaea pinnatifida Cass., is one of the species of one such traditional Indian Ayurvedic herb known as Gojihva. As per the Ayurvedic and modern research, Gojihva possesses hepatoprotective, diuretic, galactagogue, and blood purifying properties. newlineAim and Objectives: The present study was aimed to investigate the medicinal hepatoprotective claims of Launaea pinnatifida plant and to isolate, characterize and estimate a marker compound therefrom by employing Bioactivity-guided isolation, so that a detailed report of one of the species of Gojihva could be obtained. newlineMaterials and Methods: The leaves and roots of the plant were separately extracted with solvents of varying polarity through successive solvent extraction technique and subjected to in vitro antioxidant and hepatoprotective bioassays, which signified that the methanol extract of leaves had significantly better pharmacological activity. Hence, the methanol extract was subjected to further isolation of the active compound. It was subjected to GS-MS and LC-MS for the identification of more phytocompounds. After vacuum liquid chromatography, it was subjected to column chromatography. Estimation of the isolated compounds was carried out by HPLC and HPTLC fingerprinting. newline