Linkage Mapping and Qtl Identification for Yield Attributing Traits Fusarium Wilt and Sterility Mosaic Disease Resistance in Pigeonpea Cajanus cajan L Millsp

Abstract

Pigeonpea [Cajanus cajan (L.) Millsp.] is one of the most important pulse crop newlinein India. Pigeonpea yield is affected by a wide variety of biotic and abiotic factors. newlineAmong these factors, fusarium wilt and sterility mosaic diseases are two of the most newlinesignificant factors that have a negative impact on yield. In order to counteract these newlinefactors, it is absolutely necessary to develop varieties that are not only capable of newlineproducing significant yields but also have the ability to withstand these diseases. The newlinepurpose of this study is to discover QTL (quantitative trait loci) regions in RIL newline(recombinant inbred lines) populations for disease resistance in pigeonpea, namely newlinefusarium wilt and sterility mosaic. Two populations were used for the study, RIL 1 newlinepopulation (Gulbarga Selection 1 × MARUTI) consisting of 346 lines for fusarium wilt newlineresistance and RIL 2 population (MARUTI × BSMR 736) consisting of 364 lines for newlinesterility mosaic disease resistance. newlinePhenotyping for yield-attributing traits was conducted at the Main Agriculture newlineResearch Station (MARS), Dharwad, during the kharif seasons of 2016 17 and 2017 newline18. Disease screening for fusarium wilt and sterility mosaic was carried out during the newlinekharif season of 2016 17 at the Agricultural Research Station (ARS) in Gulbarga newlineand ICRISAT in Patancheru, respectively. Analysis of variance revealed good amount newlineof genetic variation in both the population for all the traits. Pigeonpea superior lines newlinewhich are high yielding and resistant to fusarium wilt (RIL-313, RIL-4, RIL-345, RIL- newline46 and RIL-205) were identified in RIL 1 population, similarly in RIL 2 population high newlineyielding and resistant lines for sterility mosaic diseases (RIL-63, RIL-68, RIL-57, RIL- newline20, and RIL-59) were identified. Genotyping was carried out in ICRISAT in two newlineplatforms i.e., 56K Axiom Cajanus SNP array and Genotyping by sequencing. newlineGenotyping in 56K Axiom Cajanus SNP array platform yielded high density genetic linkage map, with 1767 mapped SNPs distributed over 11 CcLGs (Cajanus Cajana newlinelinkage groups