INTERACTION EFFECT OF RHIZOMICROBIOME AND PHYLLOMICROBIOME ON GROWTH And YIELD OF SORGHUM

Abstract

newline The present study was carried out to isolate and characterize the Rhizomicrobiome newlineand Phyllomicrobiome from different cereal rhizosphere soil and leaf sample respectively. newlineForty rhizosphere bacteria and forty phyllosphere bacteria were isolated from forty samples newlinecollected from nine villages of Kalyana Karnataka. Based on the Morphological and newlineBiochemical characterization, bacterial isolates were identified upto generic level. Among all newlinethe eighty isolates ten rhizosphere and ten phyllosphere isolates were selected based on newlinePGPR potential. These twenty isolates were screened for beneficial traits viz., phosphate newlinesolubilization, IAA production, siderophore production, ACC deaminase activity and HCN newlineproduction etc. These isolates were used for evaluation under green house condition. Based newlineon the growth parameters best two rhizosphere (CRMS 6, CRMS 28) and two phyllosphere newline(CPRMS 5, CPRMS 35) isolates were selected for molecular characterization and they were newlineidentified and confirmed as Paracoccus marcusii (CRMS 6), Flavobacterium chungangense newline(CRMS 28), Serratia marcescens (CPRMS 5) and Methylobacterium radiotolerans (CPRMS newline35). These four efficient isolates were selected as bioformulation for the field experiment newlinewith 9 treatments and 3 replications. Out of 9 treatments, T7 [(75% RDF + CRMS 6 newline(rhizosphere) + CRMS 28 (rhizosphere) + CPRMS 5 (phyllosphere) + CPRMS 35 newline(phyllosphere)] significantly superior over other treatments by enhanced the PGPR activity newlinealso showed significant increase in the nutrient uptake and enhanced the growth and yield of newlinesorghum crop inoculated with efficient consortia inoculation in combination of rhizosphere newline(CRMS 6 and CRMS 28) and phyllosphere (CPRMS 5 and CPRMS 35) along with 75% of newlinerecommended dose of fertilizer.