Bone regenerative potential of a recombinant parathormone derivative in experimentally induced critical size calvarial defects in wistar albino rats

Abstract

newline Objective: newlineTo analyze the bone regenerative potential of a recombinant newlineparathormone derivative in experimentally induced critical size calvarial newlinedefects in wistar albino rats. newlineIntroduction: newlineAutogenous bone is the most successful bone-grafting material; however, newlinemultiple disadvantages continue to drive developments of improved methods newlinefor bone regeneration. Recombinant Parathormone derivatives have been newlineused to treat osteoporosis, osteoradionecrosis of the jaw, they are said to newlinehave an anabolic nature when delivered intermittently at low doses.The newlinerecombinantly prepared fragments (1-34 amino acids) of PTH known as newlineteriparatide is said to improve the bone mineral density and improve the newlinestrength of bones. newlineMaterials and Methods: newlineCritical-size cranial osteotomy defects were created in 30 wistar albino rats. newlineThe animals were randomly divided into 5 groups. Group I is the sham newlinecontrol, where the bone defect was only filled with a blood clot. In the Groups newline10 of 99 newlineII,III and IV it was filled with varying concentrations of recombinant PTH, in newlineGroup V, the defect was filled with and#946;-TCP+HA. The bone regeneration was newlineassessed at 30 days and 60 days, post operatively by means of newlinehistopathology, histomorphometry and radiographs.At the end of the newlineexperiment period,blood was collected by puncturing retro-orbital venous newlineplexus and analyzed for serum biochemistry for toxicity. newlineResults: newlineThe histological evaluation of the critical size cranial defects, in the sham newlinecontrol group the blood clot was observed along the entire length at the top newlineof the defect. Over the dura mater there was a narrow band of connective newlinetissue. In the group II, the blood clot was seen in the upper portion of the newlinedefect and connective tissue covering the entire length of the lower portion. newlineIn group-III, the blood clot can be seen in the defect and connective tissue newlinecovering the entire length of the upper portion. In the group IV- there was newlinetrabecular bone formation at the upper part of the defect. In the group-V, newlinethere were remnants of the and#946; T