Efficacy studies on protein dna and Replicon based vaccine candidates Against white spot syndrome virus Wssv and temporal analysis of Proteomic changes in shrimp during Wssv infection as a lead for molecular Pathogenesis

Abstract

White Spot Disease WSD a major threat to shrimp aquaculture newlineindustry is caused by White Spot Syndrome Virus WSSV WSSV is a large newlinenonoccluded bacilliform enveloped dsDNA virus It first appeared in the newlineepizootic of Taiwan during the year 1992 and later spread to all shrimp newlinefarming nations around the globe The cumulative economic loss due to newlineWSSV epidemics has crossed over 8 billion dollars since its first appearance newlineSeveral studies using various vaccination and prophylactic approaches have newlineshowed promising results of shrimp protection against WSSV challenge In newlinethis study we had tried protein DNA vaccination and bimodal vaccination newlinecombination of both protein and its perspective DNA vaccine candidate newlineusing VP28 VP281 and ICP11 as vaccine candidates to achieve better newlinesurvival Unfortunately none of these approaches were found to be effective newlinein providing protection to shrimp against newline newline