Morphological and molecular characterization and effect sowing dates and seed pelleting on seed production of french bean Phaseolus vulgaris L

Abstract

seed pelleting on seed production of French bean (Phaseolus vulgaris L.) were newlineconducted during rabi/summer seasons of 2006-07 and 2007-08 in the Department of Seed newlineScience and Technology and Institute for Agri Bio-Technology, University of Agricultural newlineSciences, Dharwad. The field study conducted on effect of five dates of sowing on seed yield newlineand quality of ten French bean genotypes revealed that among the dates of sowings, first newlineweek of October sowing of DWD-FB-1, DWD-FB-57 and DWD-FB-53 genotypes recorded newlinehigher seed yield (2270,2187and 2124 kg/ ha respectively) with higher growth, yield newlinecomponents and seed quality parameters and was followed by first week of September newlinesowing. Studies on seed pelleting revealed that seeds pelleted with Borax (100 mg), ZnSO4 newline(250 mg) per kg of seed and ZnSO4 + Borax recorded higher seed yield (2412, 2247 and newline2240 kg/ha),seed recovery (94.70, 92.93 and 91.45 %, respectively), seed germination and newlineseedling vigour index. newlineMorphological characterization of French bean genotypes based on seed coat colour, newlineshape, test weight, seed length, phenological, seedling characters was found to be more newlinereliable for varietal identification. Chemical tests, viz. Phenol , Modified phenol , FeSO4, KOH newlineand NaOH tests did not show any reaction to the chemicals and hence they were found newlineunsuitable for characterization of varieties in French bean. newlineIn RAPD studies, 33 primers produced 72 amplicons, of which 53 levels showed newlinepolymorphism amongst ten genotypes. Based on sample matching co-efficient, a genetic newlinesimilarity was constructed by using RAPD data to access genetic relatedness. All the ten newlinegenotypes were grouped in three clusters. DWD-FB 9, DWD-FB-8 and DWD-FB-18 in newlineCluster-I, DWD-FB-27, DWD-FB-6 and DWD-FB-1 in Cluster-II and DWD-FB-57, newlineDWD-FB-53, IIHR-909 and Contender in Cluster-III. Genetic dissimilarity co-efficient varied newlinebetween 0.039 and 0.06. newline