efficacy of annona muricata mediated selenium nanoparticles as an anticancer agent in oral squamous cell carcinoma cell lines

Abstract

Conventional therapies including chemotherapy, radiotherapy and surgery for newlinethe management of cancer have several side effects due to their lack of newlinespecificity and potent toxicity. Due to the inadequacy of most of the current newlinetherapeutic agents in minimising the side effects the complementary and newlinealternative medicines have attracted the view of researchers and medical newlinepractitioners more recently. A. muricata, commonly known as Graviola, soursop newlinehave promising anticancer activities. With nanotechnology, selenium an newlineessential element shows antitumor properties and promtes targeted delivery, newlineOBJECTIVES newlineand#9679; Preparation of the plant extract of Annona muricata and to synthesize and newlinecharacterise the selenium nanoparticles using UV spectroscopy, SEM and newlineFTIR newlineand#9679; Characterisation of Annona muricata mediated selenium nanoparticle gel newlineby evaluating its antioxidant, anti inflammatory and cytotoxic activities. newlineand#9679; To validate the anti cancer effect of Annona muricata mediated selenium newlinenanoparticle gel by evaluating the cell viability assay using MTT assay , newlineassessing the apototic activity by Dual AO-EB staining and evaluating newlinethe free radical scavenging activity (ROS species ) - DCFDA staining newlineMATERIALS AND METHODS newlineThe study was designed to evaluate the anti cancer activity of Annona muricata newlinemediated selenium nanoparticle gel and doxorubicin individually as well as in newlinecombination in oral cancer cell lines using MTT asaay for cell cytotoxicity , newlineDual staining by AO/EB stain for apoptotic activity , free radical scavenging newlineactivity (ROS species ) - DCFDA staining in Oral squamous cell carcinoma cell newlinelines (KB-1cell lines), anti inflammatory activity by BSA and EA assay and newlinezebrafish embryonic study for toxicity evaluation in AMSeNps. newline