Protein Stabilized Bioactive Lead Sulfide and Gold Nanomaterials for their Biological Applications

Abstract

newline Precise morphologies of pH responsive bioactive lead sulfide nano particles (PbS NPs) were synthesized by using industrially important proteins like zein and lysozyme (Lys), and a bioactive polymer diethylaminoethyl dextran chloride (DEAE) and following a simple single step method monodisperse non-hybrid silica and hybrid colloidal silica of and#8804; 200 nm decorated with small Au nanoparticles (NPs) were synthesized. Non-hybrid silica NPs were synthesized in the absence and presence of different twin tail cationic surfactants, while tiny Au NPs were grown under in situ reaction conditions on non-hybrid silica synthesized previously by using cationic dextran .Seed growth (S G) method was used to synthesize gold (Au) nanoparticles (NPs) in the presence of water soluble star polymers tetronics to investigate a unique photophysical behavior of Au NPs. Zein demonstrated a fine crystal growth control of PbS NPs better than Lys as well as DEAE, and even better than conventional surfactants known for their shape control behavior. For their industrial scale uses, different extraction methods were proposed by using other industrially important biomolecules and ionic liquids. Alginic acid and xanthan gum were excellent complexing agents for an instant extraction of Lys and DEAE coated NPs from aqueous phase. newlineBoth non-hybrid and hybrid silica NPs demonstrate excellent ability to extract proteins fractions predominantly of relatively low molecular masses, i.e., ~ 80 kDa. Applicability for more complex biological fluid like serum indicated the competitive extractions among strongly versus weakly bound proteins. With significant bearing in in vivo conditions, hybrid silica was potentially toxic towards the blood cells in comparison to non-hybrid silica. It stems from the collective interactions of silica as well as nanometallic surfaces of Au NPs to interact with the blood cells causing hemolysis and hence may not be the suitable vehicles for drug release in systemic circulation. newlinexx newlineThey produced absorbance in the