evaluation on the biopotency of proteinase inhibitors on plutella xylostella linnaeus and spodoptera frugiperda

Abstract

newline Serine proteinase inhibitors produced by plants, inhibiting protein digestion in newlineherbivorous insects, can be used as one of the best ecofriendly insect management newlinetactics. In this context, laboratory assay was conducted to identify, purify and newlinecharacterize the potential source of serine proteinase inhibitors and to identify its newlineefficacy on Plutella xylostella and Spodoptera frugiperda at the Department of newlineAgricultural Entomology, Agricultural College and Research Institute, Madurai newlineduring 2017-21. newlineSeeds of 38 plants from nine families were selected and screened for trypsin newlineand chymotrypsin inhibitory activity (TIA and CIA). Out of 38 seeds screened for the newlinepresence of TIA and CIA, 20 seeds were found to possess both TIA and CIA, newline15 seeds had only TIA. This is the new report on the presence of trypsin newlineinhibitor in Cassia leptophylla, Clitoria ternatea alba, Parkia biglandulosa, newlineSolanum xanthocarpum and Tectona grandis seeds. Parkinsonia aculeata, newlinePeltophorum pterocarpum and Jatropha carcass seeds were found as source of newlinechymotrypsin inhibitor for the first time. Tephrosia purpurea and Momordica newlinebalsamina seeds were identified as both trypsin and chymotrypsin inhibitor source for newlinethe first time. newlineAmong the seeds screened, the highest mean TIA was found in the newlineseed extracts of M. balsamina (9879 TIU g-1 newlineof seed) followed by E. variegata newline(2737 TIU g-1 newlineof seed) and the highest CIA was recorded in E. variegata newline(2800 CIU g-1 newlineof seed). Hence, those two proteinase inhibitors were selected and newlinepartially purified by ammonium sulphate precipitation. After precipitation, Fraction newline(0-60) and Fraction (30-90) were selected based on their activity from E. variegata newlineand M. balsamina, respectively, for further purification in gel filtration newlinechromatography using Sephadex G-75 column. Out of 35 fractions of EvPI and MbPI newlinecollected in Gel filtration chromatography, F12 and F13 of EvPI and F17 and F18 of newlineMbPI were selected based their TIA and CIA and they were pooled, newlinedialysed and lyophilized sepa