Doxorubicin encapsulated hydroxyapatite nanoparticle and its nanocomposite with polymer for bioengineering applications

Abstract

Hydroxyapatite[Ca10(PO4)6(OH)2](HAp) is an amazing biological material which is effectively used in bone related applications for its excellent cytocompatibility, biological activity and operative osteoconduction. In fact, bone is a hard connective tissue composed of HAp crystallites which are dispersed in the naturally occurring matrix polymer, collagen. Therefore, HAp based novel nanocomposites preparation using various polymers have become a domain of great research interest. However internal bonding between the filler material and matrix part is considered as a vital parameter for nanocomposite development. In this research study the synthesis procedures of HAp and HAp based drug-polymer nanocomposites by different chemical precipitation methods were widely discussed. The prepared HAp based nanoassemblies were further physicochemically characterized by Fourier transform infrared spectroscopy X-ray diffraction transmission electron spectroscopy, particle size distribution, also the biocompatibility and the anticancer activity of the HAp and different types of HAp based drug-polymer nanocomposites were evaluated through a colorimetric assay(MTT assay). newlineThe functional groups of synthesized HAp and HAp based nanocomposites were well identified by FTIR analysis. The other results like XRD, TEM analysis signified that the use newlineof biodegradable polymers such as polyvinyl alcohol, poly lactide-co-glycolide and chitosan(CS) as matrix enables better dispersion of nanoparticles with strong interfacial bonding interactions which can enhance the physicochemical properties of the synthesized nanocomposites. Also the cell viability of synthesized HAp was found reliable and the cytotoxicity of prepared DOX-HAp-polymer nanocomposites toward cultured osteosarcoma cell (MG 63) were found outstanding. Therefore, the developed nanocomposites may be possibly used as bone substitutes as well as have the potential to inhibit osteosarcoma and explored for further orthopedic applications.