characterization and biological evaluation of adenocalymma aliaceum miers flowers for antidiabetic activity

Abstract

Background: Diabetes mellitus is a metabolic endocrine disorder due to insulin newlineimbalances associated with microvascular and macrovascular complications. newlineObjectives: This research aimed to explore the diabetes-healing effect of newlineAdenocalymma alliaceum Miers phytochemicals. Materials and methods: Before newlinesecondary screening on animals, phytochemical constituent estimation, thin-layer newlinechromatography, high-performance liquid chromatography, column chromatography, newlineGas chromatography-Mass Spectroscopy (GC-MS), and acute toxicity study were newlineperformed. Both Invitro and in vivo models were studied to confirm the antidiabetic newlineproperty of the Adenocalymma alliaceum Miers flower (EEAAF). Invitro study was newlineperformed using a rat pancreatic cell line for cytotoxicity, glucose utilization, and newlineglucose redistribution assay. Later acute toxicity as per OECD TG 423 and in vivo newlineexperiments was carried out by two diabetic inducing agents i. Streptozotocin, ii. newlineAlloxan monohydrate. In both model, Wistar albino rats was carried out in five groups newlineof animals Normal control (NC), diabetic control (DC), Standard group (Std), Low dose newline(EEAAF 100 mg/kg, b.w), Medium dose (EEAAF 250 mg/kg, b.w) and High dose newline(EEAAF 500 mg/kg, b.w) group. Each group consists of six animals. Upon regular newlineobservation for fasting blood glucose level, body weight, polyuria, polyphagia, serum newlineprotein, serum creatinine, serum urea, and lipid profile for four weeks, the animals were newlinesacrificed using Ketamine hydrochloride. Organs were removed. Pancreases were used newlinefor observing cellular injury using Olympus binocular microscope at 45X. Isolated liver newlinecells homogenate used for free radical scavenging activity such as superoxide dismutase newline(SOD), catalase (CAT), and glutathione (GSH). Results: The results showed the newlinepresence of polyphenol and alkaloids, especially flavonoids. Current characteristics of newlineA. alliaceum include many principal secondary metabolites on GC-MS. On isolation newlineand spectroscopic determination, Flacitran was found. There was neither mortality nor newlinemorb