IDENTIFICATION OF POTENTIAL INHIBITORS AGAINST E Coli MUR ENZYMES THROUGH VIRTUAL SCREENING AND In Vitro ASSAY

Abstract

Antibiotic-resistant bacteria are increasingly causing serious infections day by day, and their newlinefrequency is rising steadily. Overuse of antimicrobial agents has resulted in the emergence, newlinerecurrence, and spread of antibiotic resistance in commensal flora and targeted bacterial newlinepathogens, making it one of the main drivers of the rapid spread of antimicrobial resistance. The newlineactive export systems present in bacterial membranes, the prevention of antibiotics penetrating newlinepathogenic bacterial cells, the enzymatic degradation of antimicrobial agents, the development of newlinethick biofilms, the alteration of antimicrobial targets, and the protection of some bacterial sites of newlineaction from antibiotics are a few examples of antimicrobial resistance mechanisms. In addition, newlinemultidrug-resistant bacteria have evolved systems that allow genetic determinants of resistance newlineto be transferred from DNA to pathogenic species. The relatively limited number of antibacterial newlinemedications that are already available in the market necessitates the creation of novel newlinemedications that are targeted at carefully selected biological drug targets. Among the novel newlinetargets, the biosynthetic pathway involved in bacterial cell wall formation is a particularly newlineappealing and remarkable source of antibacterial targets. In this regard, Mur enzymes are crucial newlinefor the synthesis of bacterial cell wall and present a prime candidate for the synthesis of newlineinhibitors directed against microorganisms resistant to antibiotics newline