A comparative study of fluorescence resonance energy transfer fret in solution phase and in hybrid films prepared by LB and LBL techniques

Abstract

Fluorescence Resonance Energy Transfer (FRET) is an electrodynamic phenomenon occurs through the transfer of exited state energy from donor to acceptor. It has vast applications in the field of medical diagnostic, DNA analyses, and study of molecular level interaction and for various sensing applications. The theoretical analysis was well developed by Förster. FRET process is distance dependent between donor and acceptor. So for the FRET applications it is very important to control the intermolecular separation between the FRET pair. Introduction of negatively charged clay particles are very useful for concentrating the cationic dye molecules on to their surfaces and hence influence the FRET efficiency