Activation of Transcription Factor Brassinazole Resistant 1 Via 24 Epibrassinolide Signalling Regulates Arsenic Stress Mitigation in Oryza Sativa Seedlings

Abstract

Arsenic (As) contamination in rice poses inevitable health risks to the global newlinepopulation, particularly in countries where rice is a major dietary component. The newlinebioabsorption of As from irrigation water and soil occurs via geogenic or anthropogenic newlineactivities, which negatively affect agricultural productivity and result in limited crop newlineyields. Phytohormones, specifically brassinosteroids (BR), are indispensable in newlinepromoting plant acclimatization to ever-changing environments by transducing newlineenvironmental signals to various developmental, physiochemical, and stress responses. newlineDespite its remarkable stress-responsive nature, the mechanism of As mitigation remains newlinelargely unexplored. In this study, we aimed to understand the mechanism underlying the newlineameliorative role of exogenous 24-epibrassinolide (24-EBL), a synthetic BR, in newlinemitigating arsenate stress in rice seedlings. newlineAn in vitro germination model was optimized by amending 24-EBL and newlinesodium arsenate in MS media and observing the morphology of IR-20 seedlings. The newlinetoxicity symptoms caused by arsenate were reversed in the presence of 24-EBL, with a newlinenearly 50% increase in root length and approximately 40% enhancement in shoot length, newlineweight, and germination rate compared with stressed seedlings newline