Formulation and Evaluation of Nano Particulate Laden Topical Delivery System for Management of Melanoma

Abstract

newline Background: Melanoma is the common form of cancer and covers half of all new cancers in Western countries. In last few years, incidence of skin cancer increased rapidly worldwide. Dacarbazine (DZ) is the most widely utilized chemotherapeutic drug for treating of melanoma. DZ is poorly soluble in water, small half life time in blood, low rate of response and greater toxic effect which ultimately limit its utilization of the treatment of skin cancer. So there is a need of development of novel topic nano formulation for better action and lesser side effects. Aim: In the presented research, we evaluated the newly formulated Dacarbazine loaded nanoparticle (DZNP) and Dacarbazine loaded nanoparticle (DZNC) as a topical formulation for the treatment of melanoma. Methodology: In view of this background current study was designed for development of dacarbazine laden nanoprticle (DZNP) ans dacarbazine laden nanocream (DZNC) topical delivery system for the treatment of melanoma. Firstly DZNP was prepared. By using DZNP its cream formulation prepared for topic drug delivery for melanoma. Dacarbazine nanoparticle and its cream was evaluated for morphology, capacity of drug load, efficiency of nanoencapsulation and size of particle and Zeta potential, Transmission Electron Microscopy (TEM), determination of pH, spreadability and viscosity and in vitro drug release capacity. Its cytotoxic potential was measured by MTT assay. Results: The particle size of DZNP and DZNC was 16.3±8.1 nm AND 16.9±7.8 respectively. pH value of nanoparticle cream was found to be 6.7±0.14. Spreadability of nanocream was 55.23±3.13 g cm/sec. Nanoencapsulation efficiency of formulation was 67.4±3.5%. Drug loading capacity was 6.73 mg/10 mg of nanoparticles. IC50 of dacarbazine nanoparticle was 0.19 mg/ml while it was 0.63 mg/ml for nanoparticle cream. From the result, it can be concluded that DZNP and its cream can be effectively and compatibly used as topical formulation for the treatment of melanoma. ABSTRACT THE GLOCAL UNIVERSITY PhD