Optimizing the Production of native am Fungi with Burkholderia Arboris and Phytohormones in Substrate based Pot Cultures and its Quality Assessment Through am signature fatty Acids

Abstract

newline Dipanti Chourasiya newline(dipantichourasiyaatgmail.com) newline newlineArbuscular mycorrhizal (AM) fungi, the most common association forming with more than 80% crop plants, highly beneficial, and are widely used as microbial bio-stimulants in agriculture. The present investigation was carried out to optimize the method for mass production of native AM fungi using organic potting substrate amended with mycorrhizal helper bacteria (Burkholderia arboris) and phytohormones as bio-stimulants. The co-inoculation of B. arboris to AM sorghum pots has enhanced spore density, root colonization, glomalin content and AM-signature lipid PLFA 16:1and#969;5cis biomass raised in the organic substrate mixture under both sterilized and unsterilized conditions. To further enhance the AM production, the co-inoculation effect of B. arboris was evaluated with selected phytohormones on sorghum in a microcosm experiment using the same organic potting substrate used in earlier experiments. Amongst the selected phytohormones, triacontanol (Tria) followed by brassinosteroids (BRs) along with co-inoculation with B. arboris to AM pots had significantly enhanced the mycorrhizal colonization and spore density in comparison to uninoculated sorghum plants grown in organic potting substrate. newlineFinally, the inoculum thus produced was tested along with key phytohormones on soybean to improve its productivity. It was observed that phytohormones application increases plant growth of soybean plant as well as AM fungi biomass belowground over the control plants. The magnitude of response was varying with dose of phytohormones and mode of application. newline