Studying the role of micro rnas in tuber development in potato solanum tuberosum ssp andigena

Abstract

We have shortlisted and validated twelve miRNAs in a SD LD photoperiod dependent manner potentially involved in tuberization pathway Our detailed insilico target prediction revealed few miRNA candidates showing potential to be tested for their involvement in tuber formation pathway Based on our preliminary results we selected miR156 and miR390 for detailed characterizations We have demonstrated that miR156V Synopsis facilitates tuber formation under inductive SD conditions miR156 over expression affectsmultiple morphological traits in potato and tobacco and it is a graft transmissible signal that potentially moves long distances via phloem in potato Overall we have shown that miR156 is a graft transmissible signal that modulates tuber formation and plant architecture in potato In our studies with miR390 StCDPK1 we have demonstrated that miR390 StCDPK1 interaction in potato possibly plays an important role in tuberization pathway To our knowledge this is the first report of miRNA mediated post transcriptional control of CDPKs newline newline