Production and characterization of fungal cutinase for potential industrial applications

Abstract

An extracellular cutinase producing fungal isolate AR08 Fusarium verticillioides was isolated from soil sample, which was active in the wide range of temperature and pH.Fungal isolate AR08 utilized orange cutin and flaxseed oil as carbon source to produce cutinase enzyme. Fermentation conditions were optimized using classical one variable at a time method and statistical method to increase the production of cutinase. Purified cutinase was found to be stable at wide range of temperature and pH. It was found to be stable in the presence of various metal ions, organic solvents and surfactants. As free enzyme has some limited over immobilized enzyme therefore cutinase was immobilized on magnetic nanoparticles which make the enzyme reusable for industrial purposes. The utilization of immobilized cutinase has demonstrated its efficacy in bioscouring of cotton and wool, degradation of organophosphorous insecticide, and ester synthesis. The findings of this study suggested that immobilized cutinase is well-suited for biodegrading insecticides, facilitating bioscouring, and promoting esterification. newline