Effects of Chitosan Folate Hesperetin Anti Doublecortin Like Kinase 1 Nanoparticles on Colon Cancer ond Cancer Stem Cells in Vitro

Abstract

Cancer is a severe health problem and one of the top causes of death of morbidity and mortality in modern society. Chemotherapy medicines now used to treat colorectal cancer are ineffective and have serious adverse effects. Cancer stem cell (CSCs), like ordinary stem cells, can self-renew and develop, giving rise to the various, differentiated cancer cells that make up the majority of the tumor. CSCs are thought to be responsible for cancer cells chemo and radio resistance. Targeting CSCs is a promising cancer therapeutic approach. Recent research has shown that plant-derived chemicals can limit the growth of CSCs. Previously, DCLK 1 was identified as a marker of colon cancer stem cells. Nanotechnology based drugs can efficiently block distinct variety of CSCs through addressing particular biomarkers. In this research, we created an especially effective nano formulated hesperetin for CSCs. The results of this study will provide information on targeting CSCs with bioflavonoids, resulted in modifications to present cancer therapeutic approaches. The bioflavonoid hesperetin is obtained from citrus fruits that has limited therapeutic value due to its low water solubility. In many cancer cells, the folate receptor is overexpressed. As a result, we covalently linked folic acid molecules with chitosan molecules to create chitosan folate hesperetin nanoparticles (CFH). CFH NPs are around 450 nm in size, this is beneficial for passively targeting cancer cells precisely this because of the tumor permeable vascular newline