Comparative pharmacognostical phytochemical and pharmacological evaluation of stereospermum colais buch ham ex dillw mabberley and stereospermum suaveolens roxbdc

Abstract

The plants selected for this study Stereospermum colais and Stereospermum suaveolensare named as Patala in Ayurvedic medicine The roots of Patala have rich traditional value especially in the treatment of inflammation and rheumatism also one of the ingredients in Dasamoola Even though Patala is a reputed Ayurvedic plant used for various ailments, there is a lacunae in the authentication and identification of the root of the two plants and the best species having antiarthritic potential Hence the aim of the study was to compare the pharmacognostical phytochemical and pharmacological studies of the roots of Stereospermum colais and Stereospermum suaveolens. Roots were collected and authenticated The pharmacognostical parameters physico-chemical analysis and elemental analysis were carried out to standardize the roots DNA fingerprint of the plants were taken using ITS2 markers Successive solvent extracts of both the plants were prepared Preliminary phytochemical analysis and fluorescence analysis of powdered root materials and various extracts were carried out which provides information regarding the presence of specific phytoconstituents in each species Quantification of primary and secondary metabolites were done and HPTLC fingerprinting of various extracts of roots of both species were taken which helps in authentication of the specific species on comparison of the fingerprints Ethanol extract of both plant were standardized using lapachol by HPLC method In vitro antioxidant in vitro anti arthritic HRBC membrane stabilization assay were carried out using various extracts of both species In vitro anti inflammatory activity of ethanol extract of both plants was screened by TPA induced inflammation in polymorphonuclear leukocytes Both the extracts showed efficacy against TNF Cathepsin D levels and nitric oxide synthase activity Stereospermum colais was found to be potent than Stereospermum suaveolens In vivo acute toxicity and In vivo antiarthritic activity using Complete Freunds Adjuvant induced arthritic model was