Genetic model using selected polymorphisms in indian boxers

Abstract

AIM: To investigate the association of and#945;-actinin-3 (ACTN3) R577X and angiotensin converting enzyme (ACE) I/D gene polymorphisms and compare it with the endurance athletes, power/speed athletes and nonathletes. newlineMETHODS: For the purpose of the study 239 male Indian subjects (57 elite boxers, 44 elite endurance athletes, 40 elite power/speed athletes and 98 non-athletic controls) were recruited. 2-3 ml saliva from the subjects was collected as sample for isolation of DNA. Simple PCR method was used to observe the polymorphism in ACE gene and PCR-RFLP method was used to observe the polymorphism in ACTN3 gene. The association of the genotype and alleles was checked by chi-square and the probability of an individual with certain genotype was found by logistic regression. newlineRESULTS: The significant association was found for R allele and RR genotype of ACTN3 gene with the status of being athlete or nonathlete (p lt 0.05). Significant difference between the allele frequency of boxers and nonathletes was found with the proportion of R allele being higher in boxers than the nonathletes (p lt 0.05), although, the difference was not significant for genotypic frequency (p gt 0.05). The odds of an individual with the RR genotype were more than twice for becoming an athlete as compared to nonathlete in X allele dominant (RX+XX) model. In case of boxers, the odds were more than thrice for possession of RR genotype in comparison to nonathlete according to same model. It was seen that endurance athletes has also significantly higher odds if possessed RR genotype in X allele dominant model. The ACE I/D gene polymorphism did not show significant association with any of the athletic groups. newlineCONCLUSION: The findings from the selected participants suggest that the ACE I/D and ACTN3 R577X gene polymorphisms did not differ between athletic groups. The R allele is associated with the status of being boxer in Indian population as compared to nonathlete.