Production of Bioplastics from Textile dyes degraded microbial Biomass

Abstract

Novel bacteria Providencia sp. RMG1 and Bacillus sp.RMG2 were isolated from industrial dye effluent and characterized based on morphological, biochemical and 16s rRNA gene sequencing. Effluent decolorization efficiency and PHA production of consortium of these microorganisms was found 85.64% and 1.45 g-l respectively. Percent dry weight of PHA was found 48.38% of total biomass after decolourization. Induction in the activity of lignin peroxidase and azoreductase was observed during decolourization of Reactive Red 4E8Y5 in the batch culture, which represented their role in degradation. Acrylic acid was used as an inhibitor for intermediate of and#946;-oxidation pathway of fatty acid biosynthesis for increasing the PHB production. PHB was extracted from bacterial cells and estimated by crotonic acid assay. At 370C, pH 5 and 2.2and#956;l/50 ml concentration of acrylic acid, 38.2 % PHB production was observed for Provedentia sp. RMG1 and at same temperature and pH and 7.5and#956;l/50ml concentration of acrylic acid maximum production of 57.73% by Bacillus sp.RMG2 which was significantly higher than PHB produced at other concentration of acrylic acid. The intermediate, metabolites have been analyses by UV-Visible spectroscopy, TLC and FTIR, techniques. Phyto toxicity of metabolites and original dye were performed and analysed. Different agricultural waste like whey waste, molasses, bagasse, rice bran were used as a substrate and studied for the production of newlineIII newlinePHB in the presence of dye. Molasses and whey waste were shown the higher production of PHB at optimum pH7 and optimum temperature 37oc and 30oc, respectively under static anoxic condition. PHB was extracted from bacterial cells and estimated by crotonic acid assay. PHB synthase activity was also detected in dye degraded microbial biomass. Extracted PHB was characterized by HPLC, FTIR, GCMS and thermo gravimetric analysis. Antimicrobial activity of PHB and montmornolite organo clay bio-composite was studied against Escherichia coli and Staphylococcus aure microbial culture.