Evaluation Of Efficacy Of Potentiated Brahmi Ghrita Using Animal Models

Abstract

Context: Sidhha ghrita is the foremost drug of choice for preventive as well as curative treatment for conditions involving cognition. However, the prime concern regarding siddha ghrita is poor drug compliance due to its quite high dose, taste, smell, palatability, it can be overcome by the process of potentiation which was tested in the present study. newlineAim: To study the relationship between drug potentiation and reduction of dose or duration or both with a case study of brahmi ghrita in chronic unpredictable mild stress (CUMS) induced memory impairment and diazepam induced amnesia models. newlineMaterials and Methods: Self prepared ekapaki (1x), panchapaki (5x) and dashapaki (10x) brahmi ghrita (BG) were standardized using HPTLC analysis with bacoside A as a biomarker. Standardized 1x BG (3.6 g/kg) and (5.0 g/kg) and 10x BG (3.6, 1.8 and 0.9 g/kg) and (5.0, 2.5 and 1.25 g/kg) were evaluated in CUMS induced memory impairment in rats and diazepam induced amnesia in mice respectively. newlineResults: HPTLC results displayed that concentration of bacoside A in the three formulations was found increased as the number of paka increased (10x BG gt5x BGgt1x BG). One-way ANOVA followed by Post hoc analysis was used for pre-clinical study. In CUMS model, 28 days exposure to CUMS significantly (P lt 0.05) reduced the percentage of sucrose consumption when compared with the non-stressed group. 10x BG at all 3 dose levels showed significantly better results (P lt 0.05) in comparison to 1x BG in memory test. In diazepam model, 10x BG did not show significant results at half and one fourth of the therapeutic dose in comparison with 1x BG on day nine. 10x BG (all three dose levels) displayed significantly positive results (P lt 0.01) compared to 1x BG on day seventeen. newlineConclusion: Drug potentiation leads to increase in the concentration of biomarker in the final formulation. 10x BG has displayed significant increase in efficacy in both the animal models.