Molecular tagging for fibre strength in cotton

Abstract

An investigation was carried out at ARS, Dharwad Farm during Kharif 2004-05 and newline2005-06 with the objectives to identify recombinant lines (F7 and F8) with hirsutum newlinemorphological and yield features and barbadense fibre properties from interspecific cross i.e., newlineDS-28 x SB(YF)-425 and to identify molecular marker(s) linked to fibre strength. newlineOn the basis of twelve easily distinguishable morphological traits, 1271 F7 plants were newlinemade into nineteen groups which are recombinant for either one or other character of both newlinehirsutum and barbadense type. Highest (1033) number of plants resembled hirsutum parent newlinewith halo length ranging from 18.00 to 37.33 mm have been isolated. Twenty plants varying newlinefor halo length from 18.00 to 37.33 mm were advanced to F8 generation. newlinePresence of low variability (range) in halo length within each progeny indicates the newlinefixation of fibre length in F8 generation. Progenies with fibre strength of 17.6 to 29.9 g/t, fibre newlinelength of 21.0 to 34.1 mm and micronaire of 2.1 to 5.5 were identified in F8. Six plants could newlinemeet the requirements in five classes of spinning potential viz., 61-80, 51-60, 41-50, 31-40 newlineand 6-12 counts. Balanced recombinants with superior fibre properties and high yielding newlineability identified in this study would be useful to develop long and extra long staple hirsutum newlinevarieties/hybrids. newlineFibre strength of 80 F2 plants ranged from 18.1 to 29.1 g/tex, with a mean of 23.1 newlineg/tex while fibre length varied from 21.5 to 35.1 mm. Only 28, out of 187 decamer primers newlineshowed consistent polymorphism between the parents. On Bulked Segregant Analysis, only newlineone primer i.e., OPC 06 amplified 700 bp fragment in bulked DNA sample of high fibre newlinestrength as well as male parent. Linkage analysis showed the presence of the linkage newlinebetween fibre strength locus and marker, with a distance of 6.25 cM. newline