esign and fabrication of biosensing devices using peptide and nucleic acid based strategies

Abstract

Biosensors have been extensively accepted by mankind for the detection of food toxins, environmental monitoring, and clinical diagnostics. Considering the countless advantages of nucleic acids and peptides over traditional bio-receptors such as antibodies, enzymes, and whole cells, the present work aims at the development of nucleic acid-based biosensors and portable biosensing devices. After that, efforts were made to combine the advantageous properties of peptides with nucleic acids for the fabrication of highly stable, sensitive, selective, and reproducible biosensors. Thus, the present thesis is devoted to novel and facile biosensing strategies based on nucleic acids and peptides. After that, the employment of developed sensing strategies was carried out in portable device fabrication for on-site detection of various moieties such as environmental toxins and biomolecules. The initial section of the work describes the peptide incorporating nucleic acid-based colorimetric method that was developed for a blacklist pollutant bisphenol A. After that, the nucleic acid-based fluorescence biosensor for the detection of arsenic ions in environmental samples was developed. Following this, the nucleic acid-based fluorescence and colorimetric biosensing method was upgraded into a small hand-held portable device for the monitoring of thrombin protein. In the final section, peptide and nucleic acid were combined to fabricate a smartphone-based colorimetric device to monitor the highly toxic lead ions in real-world samples. newline