Investigation on Bio agents and their Role in Management of Root knot Nematode Meloidogyne incognita Kofoid and White 1919 Chitwood 1949 Infecting Tomato Lycopersicon esculentum Mill

Abstract

newline Root-knot nematode (RKN), Meloidogyne incognita is a serious pest on newlinevegetables and is a major limiting factor especially in the commercial production of newlinetomato in many parts of the world. The chemical approach for management of the newlinenematode has negative impacts on beneficial microorganisms associated in soil and newlinecontributing the source of environmental pollution. Therefore, eco-friendly newlineapproaches (use of bio-control agents with organic amendments) for the management newlineof RKN has received much attention during recent years. newlineThe present study was conducted to evaluate different bio-control agents for newlinehatching inhibition and juvenile mortality of root-knot nematode, M. incognita under newlinelaboratory condition. Among the different bio-agents tested, Purpureocillium newlinelilacinum and Trichoderma viride (106 cfu/ml) was showed highest egg hatching newlineinhibition and juvenile mortality of M. incognita after 120 hours respectively. newlineDifferent bio-control agents were assessed their enzymatic activities against newlineRKN infecting tomato. Among all the tested fungal bio-agents, T. harzianum with SA newline(5g/kg soil) and ST (5g/lit of water) was showed highest biochemical activities of PO, newlinePPO and PAL content as well as biometric parameters after 15, 30 and 45 DAI and newlinesignificantly decrease in nematode multiplication at 30 and 45 DAI. newlineAn experiment was conducted in field to examined the fungal and bacterial newlinemicrobiome in tomato rhizosphere augmented bio-agents challenged against RKN at newlinetaxonomic levels. Composition of the fungal and bacterial microbiome in tomato newlinerhizosphere treated with T. harzianum and P. lilacinum were significantly higher in newlinethe all soil samples and exhibited the maximum biometric parameters and significant newlinereduction in the RKN. Furthermore, the potential fungal bio-control agents were newlinetested both in cage house and field condition, the treatment of T. harzianum with SA newline(5kg/ha.) and ST (5g/lit of water) was found most effective in suppressing RKN and newlineincreasing biometric parameter of t