Biodiversity and Conservation of DACTYLORHIZA HATAGIREA D DON Soo from Trans Himalayan Ladakh Region of India

Abstract

The morphometric study was conducted during 2009 to 2010. About 28 morphological characters were measured under 13 natural locations of D. hatagirea D. Don Soo. Geographic variation in morphology reflects phenotypic responses to environmental gradients and evolutionary history of populations and species. At points beside its broad geographic range Nubra Suru and Indus valley characterization of Dactylorhiza phenotype was normally accomplished by use of morphological descriptors hence as a first step phenotype collection and its morphometric analysis was assessed for the first time. However plant height leaf length lowermost leaf length length of second leaf from base and mean length from lowest bract to the top of inflorescence were presented to account for the remarkable variation in morphological traits. Tirith location showed more values of this trait while Skurru showed less value. From this it was concluded that Tirith showed great morphometric variation as compared to other location.RAPD and ISSR marker analysis have been used for the first time to characterize the population genetic structure and differentiation within and among thirteen locations of D. hatagirea. The genetic diversity of D. hatagirea has been revealed by Nei s diversity index (H) Shannon s diversity index I polymorphic loci and percentage of polymorphic loci (PPL). Pairwise location genetic distances ranged from 0.05 to 0.48. Although both the molecular markers revealed high percentage of polymorphism ISSR marker detected more diversity than RAPD marker. Analysis of molecular variance (AMOVA) revealed that 57% RAPD and 60% ISSR variability was partitioned among population with moderate level of genetic differentiation and gene flow. newline