Peptide therapeutic based nanoformulations synthesis stability studies and biological evaluation

Abstract

The present thesis includes the synthesis, and characterization of peptide and drug-loaded nanoformulations using Fourier Transform Infrared Spectroscopy, Field Emission Scanning Electron Microscopy, Dynamic light Scattering, and Zeta potential analysis. Further, the nanoformulations were evaluated for drug release studies and in-vitro cytotoxicity assay. The study of various nanoformulations included the investigation of the antileukemia peptide PR1 encapsulated fluorescent quantum dots (QDs). QDs decorated with RGD peptide were also synthesized and tested for delivery of doxorubicin and bioimaging applications. Furthermore, doxorubicin-encapsulated chitosan-based polymeric nanoparticles, cholesterol-based solid lipid nanoparticles, chitosan-coated poly(methacrylamide), and rylene dye-based fluorescent nanoformulations were examined. Nanoparticles embedded in chitosan hydrogel were studied as a versatile hybrid system for drug delivery applications. Moreover, nanoparticles were explored for the encapsulation of highly sensitive energetic propellant. Employing an encapsulation strategy, the present thesis assessed the therapeutic potential of different nanoformulations for efficient therapeutic agent delivery and successful storage of energetically active compounds. newline