Analyses of transcription factors from pathogenic organisms elucidation of their involvements in disease developments

Abstract

The main goal of the thesis titled and#8213;Analyses of transcription factors from pathogenic newlineorganisms - elucidation of their involvements in disease developmentsand#8214; is to analyze the newlinemechanism of DNA-protein interactions and how the DNA-protein interactions are newlineassociated with diseases. DNA-protein interactions play vital roles in many of the newlinebiological processes. The central dogma of molecular biology relies on the proper binding newlineinteractions between the DNA and the protein [1]. The binding of proteins to DNA lead to newlinethe generation of different types of virulence factors from pathogenic organisms. For my newlinestudy, I used the pathogenic organism Pseudomonas aeruginosa. Pseudomonas aeruginosa newlineis an opportunistic human pathogen [2]. The organism attacks immune-compromised newlinepatients and spreads secondary infections [3]. The severities of the infections are such that newlinenearly 60% of deaths in hospitals are caused due to secondary infections by the organism newline[4]. The organism codes for highly poisonous hydrogen cyanide (HCN) by the enzyme newlineHCN synthase. The enzyme is transcribed by the genes of the operon hcnABC [5]. The newlinetranscription of the genes of the operon is mediated by three proteins called LasR, ANR and newlineRhlR [6, 7]. The genetics of the aforementioned transcription process is well understood. newlineHowever, till date, the molecular mechanism of the process is not known. In my thesis newlinework, I tried to analyze and predict the molecular mechanism of the transcription process as newlineit has not yet been deciphered. Therefore, I extracted the existing data pertaining to the newlinehcnABC operon and used and analyzed them with the help of existing bioinformatics tools to newlinecome up with a suitable computational model to interpret the molecular mechanism of the newlinesynthesis of HCN from hcnABC operon newline