SYNTHESIS BIOACTIVITY OF 2HCHROMENE BASED FUNCTIONALIZED MOLECULES AND INCLUSION COMPLEX OF NITROCHROMENE WITH betaCD

Abstract

Heterocyclic molecules implanted with nitrogen and oxygen atoms have been used in diverse biological and pharmacological applications [170]. Among different types of nitrogen and oxygen-containing heterocyclic compounds, five-membered heterocyclic compounds like azole, thiazole, oxadiazole, triazene, imidazole, purines, pyrazole etc. play a very important role in human life due to their medicinal action against several diseases [171-178]. Specifically, oxadiazole based compounds were found to exhibit anti-inflammatory, antimicrobial, antitubercular, antidepressant, antimalarial, anti-HIV, and anticancer properties [179-188]. Due to their biological activities, the synthesis of novel oxadiazole compounds is the current research area of interest to produce analgesics, antispasmodics and anti-inflammatories. Recently, our research group reported the microwave-assisted synthesis of chromene based 1,2,4-oxadiazole molecules having potent antibacterial activity [189]. Also, as reported earlier these oxadiazole nuclei contribute significant bioactivity due to their lipophilic nature and have the ability to bind with the target protein through hydrogen bonding [190]. Protein molecules play a crucial role in the effective action of several drugs in living beings [191]. The pharmacodynamics and pharmacokinetics of drugs generally depend on the binding of these molecules with albumin proteins [192-194]. Therefore, studying the binding mechanism responsible for the molecular interactions between drug molecules and albumin could help design new bioactive molecules. We selected bovine serum albumin (BSA) as a model protein for this study as it is largely present in the blood plasma and plays a vital role in the transportation of several endogenous and exogenous ligands (such as fatty acids, amino acids, steroids, and various drugs) [195]. Also, BSA is structurally similar (around 76%) to human serum albumin (HSA). It consists of 583 amino acid residues with a molecular weight of 66 kDa. BSA structure can be divided into three