development and characterization of cell line from catfish pagasianodon hypophthalmus and its application as an in vitro tool for toxicological studies

Abstract

newline Development and Characterization of Cell Line from Catfish newlinePangasianodon hypophthalmus and Its Application as an In Vitro Tool for newlineToxicological Studies from India newlinePankaj Soni newlineAbstract newlineResearch problem newlineAquaculture and fisheries have been developed at a good pace in the last two decades newlineworldwide. This becomes possible due to evolving culture practices and scientific newlinecontributions. On a global scale, fish farming contributes significantly to people s livelihood newlineand nutrition in coastal regions. Although in India, the culture of the Indian major carp newlineconstitutes a bigger part of fish farming, the species Pangasianodon hypophthalmus has newlinegained great importance. Since the introduction of P. hypophthalmus in 1997 in India, it has newlinebecome very popular amongst fish farmers. This species is now being cultured on a large newlinescale in India. The culture of P. hypophthalmus is easy, and a high-density culture is achieved newlinein less time due to its fast growth rate. However, there is an associated risk of pathogenic newlineinfections. Besides this, increasing water pollution is a serious issue for fisheries in India newlineand other parts of the world. People who are dependent on fish as their dietary protein source newlineare facing the danger of increasing heavy metal content and other ecotoxicants in water. This newlineneeds immediate attention to find a solution for the issue that will help to assess and eliminate newlinethe risks associated with P. hypophthalmus culture. newlineTo address this, the present study aims to develop and characterize the cell lines from newlineP. hypophthalmus and utilize them for toxicological studies with several other in vitro newlineapplications, too, as ethical issues, reproducibility of results, cost, and ease are big hurdles newlinein studies based on the whole animal. newlineMethodology and Findings newlineCell lines offer an alternative approach for pursuing scientific research and newlineunderstanding viral pathologies. However, the mechanism of such infections is poorly newlineunderstood due to the lack of host-specific cell lines. To br