Isolation and identification of bioactive compounds From various extracts of seagrasses against ocular Pathogens

Abstract

newline The eye is a unique organ which gets infected from external sources or through newlineinternal sources of micro-organisms carried in person and causes many eye infections such as newlineconjunctivitis, blepharitis, scleritis, etc., Ocular medications such as eye drops or an ointment newlineare used to treat and prevent eye diseases. Many traditional herbal eye drops are prepared newlinefrom many medicinal plants combination, could cure ophthalmic disorders. External ocular newlineinfections are among the leading causes of ocular morbidity and blindness in developing newlinecountries. Drug discovery from marine ecosystem possess numerous complex products and newlinenovel chemical entities were isolated from sponges, coelenterates, tunicates, mollusks, newlineechinoderms, seagrass, seaweeds and bryozoans. Seagrasses are submerged marine newlineangiosperms grows in tidal and sub tidal areas of all ocean and it is used as human food newlinebycoastal populations.Traditionally, seagrasses were used for the treatment of fever, skin newlinediseases, muscle pains, stomach problems, heart Problems, sea-sickness. From the above newlinefacts it is clear that there only few reports are available regarding antibacterial, antialgal, newlineantifungal and antiviral activities of the seagrass. Therefore this present investigation was newlinejustifiably planned to isolate and identify the bioactive compounds, from various extracts of newlineseagrasses, against ocular pathogens. In this study, 128 isolates were isolated from 84 patients newlineof both male and female from Faith Multispecialty Hospital, Chennai. From the conjunctival newlinesmear, out of 16 bacteria, 9 predominant bacteria were selected and were taken for further newlinestudy. Among four solvent extracts of the seagrasses tested, chloroform and ethylacetate newlineextracts of Halophila ovalis exhibited significant antibacterial activity against four ocular newlinepathogens, out of nine ocular pathogens. From the proximate analysis it is found that newlineseagrasses have essential nutritional value and it can be used as diet also. The phytochemical newlinescreening showed the presence of phenol, steroi