Cloning expression purification biochemical structure characterization and application of and#945; L arabinofuranosidase PsGH43 12 of family 43 glycoside hydrolase from Pseudopedobactor saltans

Abstract

Hemicellulose is the second most abundant polysaccharide present on earth. The presence of arabinose side chains in hemicellulose such as xylan interact with other polysaccharides and forms complex network resulting in the recalcitrant nature. α-L-Arabinofuranosidases hydrolyses the arabinose side chain and having immense industrial importance. PsGH43_12 is the first α-L-arabinofuranosidase from Pseudopedobacter saltans belonging to family 43 glycoside hydrolase cloned and expressed. It showed